Detection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays
Author Department
PVLSI
Document Type
Article, Peer-reviewed
Publication Date
7-1-2009
Abstract
Rapid and effective differentiation between normal and cancer cells is an important challenge for the diagnosis and treatment of tumors. Here, we describe an array-based system for identification of normal and cancer cells based on a "chemical nose/tongue" approach that exploits subtle changes in the physicochemical nature of different cell surfaces. Their differential interactions with functionalized nanoparticles are transduced through displacement of a multivalent polymer fluorophore that is quenched when bound to the particle and fluorescent after release. Using this sensing strategy we can rapidly (minutes/seconds) and effectively distinguish (i) different cell types; (ii) normal, cancerous and metastatic human breast cells; and (iii) isogenic normal, cancerous and metastatic murine epithelial cell lines.
Publication ISSN
0884-8734
Recommended Citation
Bajaj A, Miranda OR, Kim IB, Phillips RL, Jerry DJ, Bunz UH, Rotello VM. Detection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays Proc Natl Acad Sci U S A 2009 Jul;106(27):10912-6.